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KMID : 0355820180390010009
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Korean Journal of Oral Anatomy
2018 Volume.39 No. 1 p.9 ~ p.17
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Postembedding Immunocytochemical Analysis of Tooth Pulp Afferent Terminals in the Trigeminal Nucleus Oralis
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Son Jae-Youn
Han Hye-Min
Kim Tae-Heon
Bae Yong-Chul
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Abstract
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Trigeminal nucleus oralis (Vo) receiving nociceptive information from the dental pulp is involved in reflexive jaw movement. However, little information is available regarding the neurotransmitters which are involved in the transmission and processing of pulpal nociceptive information in the Vo. To address this issue, we labeled afferents of rat upper molar pulps using retrograde wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) tracing, performed postembedding immunocytochemistry with glutamate and GABA antisera, and ultrastructural analysis. The labeled pulpal afferent terminals in the Vo contained many spherical synaptic vesicles (45-55 nm in diameter). They frequently made asymmetrical synaptic contacts with the middle or distal parts of dendrites rather than the soma or proximal part of dendrites. They received synapse from pleomorphic vesicles containing presynaptic endings. Gold particles labeling the glutamate were densely distributed in the afferent terminals of the tooth pulp but were sparse in the presynaptic endings. On the other hand, gold particles labeling GABA were densely distributed in the presynaptic endings, but sparse in the afferent terminals of the tooth pulp. These findings indicate that tooth pulp afferent terminals use glutamate as a neurotransmitter in the Vo and they are controlled by presynaptic endings that use GABA as a neurotransmitter.
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KEYWORD
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pulpal afferent, synaptic connectivity, presynaptic inhibition, GABA
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